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目的采用高效液相色谱法建立两面针的HPLC色谱指纹图谱分析方法,为其品质控制提供可靠依据。方法采用HPLC-UV分析两面针的指纹图谱。色谱柱:UltimateTMXB C8柱(250 mm×4.6 mm,5μm);流动相为水(0.2%磷酸+0.2%三乙胺)(A)-乙腈(B),梯度洗脱:0～5 min,8%～12%B;5～7 min,12%～15%B;7～14 min,15%～21%B;14～35 min,21%～25%B;35～40 min,25%～36%B;40～60 min,36%～52%B;60～80 min,52%～100%B;80～95 min,100%B;体积流量1.0 mL/min;柱温35℃;检测波长250 nm,测定24批两面针指纹图谱,应用相似度分析、系统聚类分析,对两面针进行分类研究。结果在色谱指纹图谱中,确定了22个共有峰,根据相似度分析、系统聚类分析的结果,将24批药材分为2类。结论该指纹图谱检测方法方便,重现性好,可用于两面针质量控制。
OBJECTIVE To establish HPLC chromatographic fingerprinting method of two-sided needle by HPLC and provide a reliable basis for its quality control. Methods The fingerprint of two-sided needles was analyzed by HPLC-UV. Column: UltimateTMXB C8 column (250 mm × 4.6 mm, 5 μm); mobile phase consisted of water (0.2% phosphoric acid + 0.2% triethylamine) % ~ 12% B; 5-7 min, 12% -15% B; 7-14 min, 15-21% B; 14-35 min, 21-25% B; 35-40 min, 25% 36% -52% B; 60-80 min, 52-100% B; 80-95 min, 100% B; volumetric flow rate 1.0 mL / min; Wavelength 250 nm, determination of 24 batches of pinprick fingerprints, the application of similarity analysis, system clustering analysis, the classification of two-sided needle. Results In chromatographic fingerprinting, 22 common peaks were identified. According to the results of similarity analysis and cluster analysis, 24 batches of herbs were divided into two groups. Conclusion The fingerprint detection method is convenient, reproducible, and can be used for quality control of two-sided needle.