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在实验室条件下通过秸杆还土试验比较了克螟稻Bt基因表达最高期秸杆和同一时期亲本稻秸杆的添加对淹水土壤可培养厌氧细菌数量和细菌群落组成的影响 .结果表明 ,与亲本对照相比 ,培养初期克螟稻秸杆的添加对淹水土壤厌氧发酵性细菌、产氢产乙酸细菌、反硝化细菌和产甲烷细菌的数量产生了显著性影响 ,但培养后期这种显著性差异基本消失 .PCR 变性梯度凝胶电泳 (DGGE)指纹图谱和主成份分析 (PCA)结果表明 ,两种秸杆处理土壤细菌群落组成在培养的第 3周和第 5周达到显著性差异 ,随着培养时间的延伸 ,两种秸杆处理土壤间细菌群落组成的差异逐渐减小 .到培养的第 1 1周 ,两种秸杆处理土壤间细菌群落组成的差异基本消失 .尽管如此 ,在培养的整个过程中 ,秸杆处理土壤中可培养厌氧性细菌数量和土壤细菌群落的组成均与纯土对照存在显著性差异 .试验结果表明 ,在实验室培养的条件下 ,没有观察到转Bt基因克螟稻秸杆对淹水土壤微生物明显的长期负面影响
Under laboratory conditions, straw-returning soil test was used to compare the effects of straw with Bt gene expression peak and rice straw in the same period on the number of anaerobes culturable and bacterial community composition in flooded soil.Results The results showed that compared with the parental control, the addition of Kilo-straw had a significant effect on the anaerobic fermentative bacteria, hydrogen-producing acetogens, denitrifying bacteria and methanogenic bacteria in the initial stage of culture, but the culture The significant difference disappeared at the later stage.PCR results of PCR denaturing gradient gel electrophoresis (DGGE) indicated that the bacterial community composition of the two straw treatments reached the third and fifth week of culture Significant differences, with the extension of incubation time, the differences of bacterial community composition between the two straw treatments decreased gradually.During the first week of culture, the difference of bacterial community composition between the two straw treatments disappeared. In spite of this, there was a significant difference in the number of anaerobic bacteria that can be cultivated in straw treated soil and the composition of soil bacterial community in the whole culture process compared with pure soil control. The results showed that under the laboratory culture conditions, no obvious long-term negative effects on Microbial population of flooded soil were observed with transgenic Bt rice straw