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研究癌基因及抗癌基因在喉癌及癌旁不同距离处的表达特点及意义。方法：应用ＬＳＡＢ微波 免疫组化法，检测了ｒａｓ，ｃ－ ｍｙｃ及 ｐ５３在 ３０例喉癌、边缘区、癌旁 ０．５ｃｍ、１．０ｃｍ、１．５ｃｍ、２．０ｃｍ处粘膜和４例正 常喉粘膜中的表达。结果；①ｒａｓ、ｃ－ｍｙｃ、ｐ５３在喉癌组织中有较高的阳性表达率，分别为８６．７％（２６／３０）、９６．７％ （２９／３０）、６３．３％（１９／３０），与癌旁及正常组织相比，差异显著（Ｐ＜０．０１），ｒａｓ、ｃ－ｍｙｃ在正常组织中有低量表达，ｐ５３ 从癌旁１．０ｃｍ处开始出现表达。②两种以上癌基因蛋白产物同时表达阳性者在喉癌组织为２８例（９３．３％），其中 ｒａｓ＋ｃ－ｍｙｃ＋ｐ５３者为１７例（５６．７％）。癌旁组织中也存在着复合表达，在正常组织中无一例联合表达。③ｒａｓ、ｃ －ｍｙｃ、ｐ５３的单独及联合表达依正常粘膜→癌旁２．０ｃｍ→１．５ｃｍ→１．０ｃｍ→０．５ｃｍ→边缘区→癌依次增高，多 数在癌旁１．０ｃｍ处与０．５ｃｍ以内处的表达存在有显著差异（Ｐ＜０．０５）。④三种癌基因产物的表达与喉癌的分化 程度无关（Ｐ＞０．０５）。结论：ｒａｓ、ｃ－ｍｙｃ、ｐ５３基因改变均参与了喉癌?
To study the expression characteristics and significance of oncogenes and anticancer genes at different distances from laryngeal cancer and adjacent tissues. METHODS: LSAB microwave immunohistochemistry was used to detect ras, c-myc and p53 in 30 cases of laryngeal carcinoma, marginal zone, 0.5 cm, 1.0 cm, 1.5 cm, 2.0 cm mucosa and 4 cases. Expression in normal laryngeal mucosa. Results: 1 ras, c-myc, and p53 were highly positive in laryngeal cancer tissues, with 86.7% (26/30), 96.7% (29/30), and 63.3% (19, respectively). (30/30), compared with paracancerous and normal tissues, the difference was significant (P<0.01). ras and c-myc were expressed in normal tissues at low levels, and p53 was expressed from 1.0 cm adjacent to the cancer. 2 The positive expression of two or more oncogene protein products was 28 cases (93.3%) in laryngeal cancer tissues, of which 17 cases (56.7%) were ras+c-myc+p53. There is also complex expression in adjacent tissues, and there is no case of joint expression in normal tissues. The expression of 3ras, c-myc and p53 alone and in combination increased from normal mucosa to adjacent tumor 2.0 cm → 1.5 cm → 1.0 cm → 0.5 cm → marginal region → cancer, most of them were located at 1.0 cm adjacent to the tumor. There was a significant difference in expression within 0.5cm (P<0.05). 4 The expression of three oncogene products was not related to the degree of differentiation of laryngeal cancer (P>0.05). Conclusion: Changes in ras, c-myc, and p53 genes are involved in laryngeal cancer.