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目的探讨抗原修复时酶消化修复法和常规微波修复法对检测结果的影响。方法取已确诊为乳腺癌患者的乳腺组织,10%甲醛溶液固定,石蜡包埋,连续切片。实验分组:随机抽取10张切片定为Ⅰ组,该组采用常规微波修复法;另取10张切片定为Ⅱ组,该组采用酶消化修复法。结果常规微波修复法,细胞染色在+～++之间,着色时间短,背景清晰。酶修复法,细胞染色介于++～+++之间,着色时间长,时间上不易控制,背景颜色较微波修复浅。结论免疫组化中酶修复较常规热修复效果理想。
Objective To investigate the effect of enzymatic digestion and routine microwave repair on the detection of antigen retrieval. Methods The breast tissues of patients with breast cancer who had been diagnosed as breast cancer were fixed in 10% formaldehyde solution and paraffin embedded. Experimental group: randomly selected 10 slices as group Ⅰ, the group using conventional microwave repair method; the other 10 slices as Ⅱ group, the group using enzyme digestion repair method. Results Conventional microwave repair method, cell staining between + ~ + +, the coloring time is short, the background is clear. Enzyme repair method, cell staining between ++ ~ +++, coloring a long time, not easy to control the time, the background color is lighter than the microwave repair. Conclusion Enzyme repair in immunohistochemistry is more effective than conventional heat repair.