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目的初步探讨蛋白酶体抑制剂PS341在治疗多发性骨髓瘤(MM)时对间充质干细胞(MSC)细胞因子白介素(IL1β)分泌的影响。方法10%胎牛血清(FBS)的1640培养液培养MSC,细胞数达0.5～1.0×106时以终浓度50nmol/L的PS341作用4h,RTPCR检测细胞因子IL1β表达。结果PS341作用后MSC分泌的IL1β表达有显著下降,完全缓解及经PS341治疗后患者的IL1β未见表达;与难治复发组比较,PS341对完全缓解患者MSCIL1β的表达抑制更明显。结论PS341抑制MM患者MSC细胞因子IL1β的表达。
Objective To investigate the effect of proteasome inhibitor PS341 on the secretion of interleukin (IL1β) in mesenchymal stem cells (MSCs) in the treatment of multiple myeloma (MM). Methods MSCs were cultured in 1640 medium containing 10% fetal bovine serum (FBS). When the number of cells reached 0.5 ~ 1.0 × 106, PS341 at a final concentration of 50 nmol / L was incubated for 4 h. RTPCR was used to detect the expression of cytokine IL1 β. Results The expression of IL1β secreted by PSCs was significantly decreased after PS341 treatment. The complete remission and the expression of IL1β in PS341 group were not observed. Compared with the refractory relapse group, PS341 inhibited the expression of MSCIL1β more significantly in patients with complete remission. Conclusion PS341 inhibits the expression of cytokine IL1β in MM patients.