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[Objective] To prepare recombinant type 1 pilus vaccine of avian Escherichia coli and to detect its immunogenicity in chickens. [Method] The type 1 pilus was respectively isolated from the recombinant bacteria CZYR10 strain and wild avian Escherichia coli YR(O18) strain and used to prepare oil-emulsified vaccine. The immunogenicity of the developed vaccine was detected in chickens using the challenge test. [Result] The recombinant type 1 pilus protein had immunoprotective efficacy. The recombinant type 1 pilus protein had weaker protective efficacy than the isolated type 1 pilus protein, but the difference was not significant. [Conclusion] The study provides a reference for immunization and control of chicken colibacillosis.
[Objective] To prepare recombinant type 1 pilus vaccine of avian Escherichia coli and to detect its immunogenicity in chickens. [Method] The type 1 pilus was individually isolated from the recombinant bacteria CZYR10 strain and wild avian Escherichia coli YR (O18) strain and used The recombinant type 1 pilus protein had immunoprotective efficacy. The recombinant type 1 pilus protein had immunoprotective efficacy. The recombinant type 1 pilus protein had weaker protective efficacy than the isolated type 1 The study provides a reference for immunization and control of chicken colibacillosis.