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为了增强免疫基因治疗瘤菌诱导机体抗肿瘤系统免疫效应,我们应用逆病毒中介基因转移技术,将IL-2、IL-4、IL-6、IL-7、IFN-α、B7-1、TNF-α、GM-CSF等多种细胞因子基因导入肿瘤细胞,证实了这些基因修饰的肿瘤细胞.由于多种细胞因子基因的导入,诱导宿主抗肿瘤系统免疫能力明显增强.在此基础上,我们为了开发增强宿主免疫的肿瘤基因治疗新方案,构建了可以表达双价细胞因子GM-CSF、TNF-α、IL-4的逆病毒载体的基因修饰瘤苗.通过接种放射线灭活的分泌GM-CSF和IL-4细胞因子的肿瘤细胞即细胞因子肿瘤疫苗,来诱导有效地抗肿瘤系统免疫功能.应用构建的双价逆病毒载体和逆病毒中介基因转移技术,对肺癌、脑肿瘤小鼠模型进行了双价细胞因子基因瘤苗的抗肿瘤免疫增强效果和
In order to enhance the immunogenicity of tumor-inducing anti-tumor system induced by immune gene therapy, we used the reverse virus-mediated gene transfer technique to treat IL-2, IL-4, IL-6, IL-7, IFN-α, B7-1, and TNF. -α, GM-CSF and other cytokine genes were introduced into tumor cells, confirming these genetically modified tumor cells. Due to the introduction of a variety of cytokine genes, the ability to induce host anti-tumor system immunity was significantly enhanced. Based on this, we To develop a novel gene therapy for tumors that enhances host immunity, genetically modified tumor vaccines capable of expressing the retroviral vectors of the bivalent cytokines GM-CSF, TNF-α, and IL-4 were constructed. Secreted GM-inactivated by inoculation with radiation Tumor cells of CSF and IL-4 cytokines, ie, cytokine tumor vaccines, induce effective anti-tumor system immune function. Application of the constructed bivalent retrovirus vector and retrovirus intermediate gene transfer technology to lung cancer and brain tumor mouse models The anti-tumor immunity enhancement effect of the bivalent cytokine gene vaccine was performed.