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目的对链霉菌CPCC 200497产生的抗菌活性次级代谢产物进行研究。方法对CPCC 200497发酵液乙酸乙酯提取物进行TLC、HPLC和MS分析,发现目标化合物,采用色谱方法分离纯化目标化合物,以高分辨质谱和NMR分析对目标化合物进行结构鉴定,并进行抗菌活性测定。结果从CPCC 200497发酵培养物中发现并鉴定主要次级代谢产物——醌霉素A和C组分,它们显示强烈的抗革兰阳性菌活性,特别是对MRSA和VRE耐药菌株的活性强于万古霉素;CPCC 200497还产生其他次级代谢产物。结论醌霉素具有显著的抗耐药菌等生物活性,有望成为抗耐药菌药物开发先导化合物;CPCC 200497产生的其他次级代谢产物有待继续挖掘。
Objective To study the antibacterial activity secondary metabolites produced by Streptomyces sp. CPCC 200497. Methods The ethyl acetate extract of CPCC 200497 fermentation broth was analyzed by TLC, HPLC and MS. The target compound was isolated and purified by chromatography. The structure of the target compound was identified by high resolution mass spectrometry and NMR analysis. The antibacterial activity . Results The major secondary metabolites, quinonecognosin A and C, were found and identified from the CPCC 200497 fermentation cultures and showed strong activity against Gram-positive bacteria, especially against MRSA and VRE-resistant strains Vancomycin; CPCC 200497 also produces other secondary metabolites. Conclusions Quinimycin has significant biological activity against drug-resistant bacteria and is expected to become a leading compound for the development of anti-drug-resistant drugs. Other secondary metabolites produced by CPCC 200497 are to be excavated.