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目的研究卡马西平对成年癫癎大鼠海马齿状回神经发生的影响。方法采用氯化锂和匹罗卡品联合诱导大鼠癫癎模型,于干预后第6d腹腔注射5-溴脱氧尿核苷嘧啶标记海马齿状回内源性神经前体细胞的增殖情况;用免疫组织化学方法及免疫荧光双标方法观察海马齿状回新生细胞的增殖、存活、分化及迁移情况。结果 (1)卡马西平可明显抑制癫癎大鼠海马齿状回新生细胞增殖;(2)卡马西平可明显促进癫癎大鼠海马齿状回新生细胞的存活;(3)卡马西平可增加癫癎大鼠海马齿状回新生神经元的数量,但不增加新生细胞分化为成熟神经元的比例;(4)卡马西平对新生神经细胞的异位迁移无抑制作用。结论卡马西平对癫癎大鼠海马齿状回神经发生的影响是其控制癫癎临床症状的可能机制之一。
Objective To investigate the effect of carbamazepine on the dentate gyrus neurogenesis in adult epileptic rats. Methods The model of epilepsy induced by lithium chloride and pilocarpine was induced in rats. The proliferation of endogenous neural precursor cells labeled with 5-Bromodeoxyuridine-labeled hippocampal dentate gyrus was detected by intraperitoneal injection on day 6 after intervention. Immunohistochemistry and double immunofluorescence staining were used to observe the proliferation, survival, differentiation and migration of hippocampal dentate gyrus cells. Results (1) Carbamazepine can obviously inhibit the proliferation of hippocampal dentate gyrus in epileptic rats; (2) Carbamazepine can significantly promote the survival of hippocampal dentate gyrus in epileptic rats; (3) Can increase the number of hippocampal dentate gyrus neurons in epileptic rats, but do not increase the proportion of newborn cells differentiated into mature neurons; (4) Carbamazepine has no inhibitory effect on the heterotopic migration of newborn neurons. Conclusion The effect of carbamazepine on hippocampal dentate gyrus in epileptic rats is one of the possible mechanisms by which carbamazepine controls the clinical symptoms of epilepsy.