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研究替米考星对3种水产致病菌体外抗菌作用,替米考星对嗜水气单胞菌(Aeromonas hydrophilia)感染异育银鲫的防治效果和替米考星对异育银鲫的急性毒性作用。应用二倍稀释法研究质量浓度为0.125、0.25、0.5、1、2、4、8、16、32、64、128 mg/L的替米考星对嗜水气单胞菌(Aeromonas hyolrophila)、温和气单胞菌(Aeromonas sobria)和鳗弧菌(Vibrio anguillarum)的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),并计算MBC/MIC比值。腹腔注射0.3 m L浓度为9×10~8CFU/m L的嗜水气单胞菌菌液感染体质量为(217.65±25.26)g的异育银鲫,分别在感染后1、24和48 h以0、50、100、150 mg/kg鱼体重的剂量分别口灌替米考星,计算替米考星对嗜水气单胞菌感染异育银鲫的保护率。应用剂量为0、260、300、340、387、440 mg/kg的替米考星口灌异育银鲫,记录异育银鲫的死亡率,计算替米考星对异育银鲫的半数致死量LD_(50)。结果表明,替米考星对嗜水气单胞菌、温和气单胞菌和鳗弧菌的MIC分别为4.00、0.50和1.00mg/L,MBC分别为64、32和32 mg/L,MBC/MIC比值分别为16、64和32;100和150 mg/kg鱼体重剂量的替米考星对嗜水气单胞菌人工感染异育银鲫的保护率可达100%;替米考星对异育银鲫口灌的24、48、72和96 h的半数致死剂量LD_(50)分别为344.0、306.3、298.5和298.5 mg/kg。替米考星对3种水产致病菌具有一定的体外抗菌效果;替米考星对嗜水气单胞菌感染的异育银鲫具有一定的保护作用;但同时替米考星对异育银鲫也具有中等毒性。本研究可为替米考星在水产养殖中的合理使用提供参考。
To study the in vitro antibacterial activity of timmoxarshin against three kinds of aquatic pathogenic bacteria, the effect of timolol on the prevention and cure of Aeromonas hydrophilia infected allogynogenetic crucian carp Acute toxicity. Two-fold dilution method was used to study the effects of timololide (Aeromonas hyolrophila) with concentrations of 0.125,0.25,0.5,1,2,4,8,16,32,64,128 mg / L, The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Aeromonas sobria and Vibrio anguillarum were calculated and the MBC / MIC ratio was calculated. Intraperitoneal injection of 0.3 m L of Aeromonas hydrophila strain with the concentration of 9 × 10 ~ 8 CFU / m L inoculated into the gilts of allogynogenetic silver crucian carp (217.65 ± 25.26 g) at 1, 24 and 48 h The doses of 0, 50, 100 and 150 mg / kg of fish were orally administered with tilmicosin, respectively. The protective rates of telmistrol to A. gmelinii infected C. heterophyllas were calculated. The dose of tamsulosin was 0,260,300,340,387,440 mg / kg, and the mortality of allogynogenetic silver crucian carp was recorded. Half of the silver trisporothrix Lethal dose LD_ (50). The results showed that the MICs of telmistrol to Aeromonas hydrophila, Aeromonas sobria and V. anguilla were 4.00, 0.50 and 1.00 mg / L, MBC were 64, 32 and 32 mg / L, MBC / MIC ratios were 16, 64, and 32, respectively. The protective rates of 100 and 150 mg / kg body weight doses of timifosine on the S. gonorrhoeae artificially infected allogynogenetic crucian carp were 100% The LD 50 of 24, 48, 72 and 96 h for Irrigation of Carassius auratus were 344.0, 306.3, 298.5 and 298.5 mg / kg, respectively. Tilmicosin has certain antibacterial effect in vitro against three kinds of aquatic pathogenic bacteria; Tilmicosin has certain protective effect on Aeromonas hydrophila infected by Aeromonas hydrophila; but at the same time, Silver crucian carp also has moderate toxicity. This study may provide reference for the rational use of timolol in aquaculture.