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利用以大赖草基因组DNA为探针的荧光原位杂交技术对导入普通小麦的大赖草染色质进行检测,结果:(1)根尖体细胞或花粉母细胞时期均可用于检测大赖草染色质。间期核中杂交信号点数与所含大赖草染色体数目之间的对应关系依染色体显强C-带末端数不同而不同;(2)利用荧光原位杂交,从普通小麦-大赖草单体异附加系的减数分裂前植株60Co-γ射线辐射后代中检测到一批大赖草端着丝粒染色体和小麦-大赖草染色体易位等结构变异;(3)用基因组原位杂交检测导入小麦的大赖草染色质时加或不加封阻DNA对原位杂交结果无太大影响。
The results showed that: (1) All the apical somatic cells or pollen mother cells could be used for the detection of Larvae Chromatin. The correspondence between the number of hybridization signal points in the interphase nucleus and the number of the chromosomes in Leymus chinensis differs according to the number of C-terminal ends of the chromosomes. (2) By using fluorescence in situ hybridization, A number of structural variants such as telomere chromosomes and wheat-Leymus chinensis chromosome translocations were detected in 60 Co-γ-ray radiation offspring of pre-meiotic lines of alloplasmic addition lines. (3) Genomic in situ hybridization Detection of introduced Leymus chinensis chromatin with or without blocking DNA on in situ hybridization results did not affect much.