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目的 观察塞替派 (TE)及环磷酰胺 (CP)能否诱发永生化人支气管上皮恶性转化细胞成瘤。方法以永生化人支气管上皮细胞 (BEAS 2B)为对照 ,以TE诱发转化细胞 (BEAS TE)的琼脂糖克隆扩增细胞(BEAS STE)及CP诱发转化细胞 (BEAS CP)的琼脂糖克隆扩增细胞 (BEAS SCP)为靶细胞接种于 3～ 4周龄裸鼠 ,每组接种 6只 ,♂♀各半。结果 接种后2 6周 ,对照组无一长出肿瘤 ,BEAS STE组有 5只长出肿瘤 ,BEAS SCP组有 1只长出肿瘤 ,其中BEAS STE组有 3只裸鼠的肿瘤直径在 1cm以上。经病理组织形态和免疫组织化学检查证实肿瘤为低分化癌肉瘤组织。结论 TE、CP诱发恶性转化的人支气管上皮转化细胞均具有裸鼠致瘤性。
Objective To observe whether thiotepa (TE) and cyclophosphamide (CP) induce tumorigenesis of immortalized human bronchial epithelial malignant transformed cells. Methods The immortalized human bronchial epithelial cells (BEAS 2B) were used as control. The agarose clone amplification products (BEAS STE) of TE-induced transformed cells (BEAS TE) and the transformed cells of CP-induced transformation Cells (BEAS SCP) were inoculated to 3 to 4-week-old nude mice as target cells, with 6 mice in each group, half in each. RESULTS: Twenty-six weeks after inoculation, no tumor grew in the control group, five tumors in the BEAS STE group and one in the BEAS SCP group. The tumors in the BEAS STE group had a diameter of more than 1 cm . The histopathology and immunohistochemistry confirmed that the tumor was poorly differentiated carcinomas. Conclusion TE, CP-induced malignant transformation of human bronchial epithelial cells have nude mice tumorigenicity.