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本文报告了从临床细胞学观点对长期体外培养的人肺癌细胞进行形态学研究的结果。发现虽然经长达一年半的体外培养,人肺鳞癌细胞系仍保留有鳞癌细胞临床诊断的标准特点。大多数细胞仍为圆形或多边形;边界清楚;核位于细胞中央;胞浆匀实;并出现典型的鳞癌细胞,如圆形癌细胞、蝌蚪形癌细胞及多核癌细胞等。在培养细胞中也出现细胞分化状态的改变,如少数细胞保存着低分化鳞癌细胞的状态,甚至呈未分化的恶性裸核,而大多数细胞转化为中分化的鳞癌细胞。值得注意的是,在少数培养细胞中出现腺癌细胞样的改变,如癌细胞的胞浆内出现单个或多个大空泡,并将核推到一边。有的细胞呈典型的恶性“印戒细胞”。因此,我们认为体外培养的人癌细胞仍可反映出人体癌细胞的临床诊断特点,所出现的差异可能是体外环境选择而使细胞改变的结果。
This paper reports the results of morphological studies of long-term human lung cancer cells cultured in vitro from a clinical cytology point of view. Found that although up to 1.5 years of in vitro culture, human lung squamous cell carcinoma cell lines still retain the standard features of clinical diagnosis of squamous cell carcinoma. Most cells are still round or polygonal; border clear; nuclear located in the center of the cell; cytoplasm uniform; and typical squamous cell carcinoma, such as round cancer cells, tadpole-shaped cancer cells and multi-nuclear cancer cells. In cultured cells also appeared changes in cell differentiation status, such as a small number of cells hold poorly differentiated squamous cell carcinoma of the state, even undifferentiated malignant nude, and most cells into differentiated squamous cell carcinoma. It is noteworthy that in a few cultured cells appear adenocarcinoma-like changes, such as cancer cells in the cytoplasm of single or multiple large vacuoles, and the nuclear aside. Some cells showed typical malignant “signet ring cells.” Therefore, we believe that in vitro cultured human cancer cells can still reflect the clinical diagnostic features of human cancer cells, the differences may arise as a result of changes in the environment in vitro selection results.