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临床和实验研究指出,脉冲磁场对组织和器官有多种作用(如止痛、抗炎、营养等),但对其机制和形态学变化并未系统和充分阐明。本文目的是用组织学和酶组织化学方法,研究脉冲磁场在实验条件下对细胞的影响。资料和方法健康豚鼠28只,体重350～400克,分为6组,用不同参数(1Hz、10Hz、160Oe、400Oe)、不同位置的脉冲磁场,单次作用10分钟。4只豚鼠不给脉冲磁场,作为对照组。在脉冲磁场作用后4小时杀鼠,取材包括肝、脾、付肾、心肌和皮肤,置10%中性甲醛、95%酒精和Carnoy 溶液固定,石蜡包埋,然后对不同组织各按不同的方法染色。低温控制切片,测定碱性磷酸酶(Gomori 法)和
Clinical and experimental studies have pointed out that pulsed magnetic fields have multiple effects on tissues and organs (such as analgesic, anti-inflammatory and nutritional), but their mechanisms and morphological changes are not systematically and fully elucidated. The purpose of this paper is to study the effect of pulsed magnetic fields on cells under experimental conditions using histological and enzymatic histochemical methods. MATERIALS AND METHODS: A total of 28 healthy guinea pigs weighing 350-400 g were divided into 6 groups. The pulsed magnetic field with different parameters (1Hz, 10Hz, 160Oe, 400Oe) and different positions were used for 10 minutes. Four guinea pigs did not give a pulsed magnetic field as a control group. Rats were sacrificed 4 hours after the pulsed magnetic field. Specimens including liver, spleen, kidney, myocardium and skin were collected and fixed in 10% neutral formalin, 95% ethanol and Carnoy solution, embedded in paraffin, and then differentiated into different tissues Method staining. Low temperature control section, determination of alkaline phosphatase (Gomori method) and