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目的观察反应停对人肝细胞癌(HCC)血管生成和肿瘤生长侵袭的干预作用。方法建立高转移潜能人肝癌裸鼠肝原位移植模型,随机分成4组。各组模型自建立次日起分别经腹腔注射0.5%羧甲基纤维素钠(CMC)、反应停(200mg/kg体重)、紫杉醇(13mg/kg体重)、反应停(200mg/kg体重)+紫杉醇(13mg/d)。给药第30天处死裸小鼠,观察肿瘤生长、转移情况,分别用免疫组织化学和半定量逆转录聚合酶链反应(RTPCR)的方法检测癌组织CD34和血管内皮生长因子(VEGF)mRNA的表达并记录微血管密度(MVD)。结果反应停组肿瘤重量、体积均与空白对照组差异无统计学意义(P>0.05)。各药物处理组肺转移灶计数、MVD和VEGF均低于对照组,以联合用药组最为显著。结论反应停对HCC生长无明显抑制作用,但能抑制肿瘤血管生成并降低肺转移。
Objective To observe the intervention of thalidomide on angiogenesis and tumor invasion in human hepatocellular carcinoma (HCC). Methods The orthotopic liver transplantation model of human hepatocellular carcinoma with high metastatic potential was established and randomly divided into 4 groups. Each model was injected intraperitoneally with 0.5% sodium carboxymethylcellulose (CMC), 200 mg / kg body weight, paclitaxel (13 mg / kg body weight), 200 mg / kg body weight Paclitaxel (13 mg / d). On the 30th day after administration, the nude mice were sacrificed and the growth and metastasis of the tumor were observed. The expressions of CD34 and VEGF mRNA were detected by immunohistochemistry and semiquantitative reverse transcription polymerase chain reaction (RTPCR) Microvessel density (MVD) was expressed and recorded. Results There was no significant difference in the weight and volume of tumors between the control group and the blank control group (P> 0.05). The lung metastasis count, MVD and VEGF in each drug-treated group were lower than those in the control group, and the combination group was the most significant. Conclusion Response to stopping has no obvious inhibitory effect on the growth of HCC, but it can inhibit tumor angiogenesis and reduce lung metastasis.