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目的 观察左旋、右旋一叶萩碱对麻醉大鼠海马齿状回基础突触传递功能的影响 ,并进一步研究其作用机制。方法 用在体记录突触传递长时程增强 (LTP)的电生理学方法 ,记录大鼠海马齿状回颗粒细胞层群峰电位(populationspike,PS)。结果 终浓度 2× 10 - 9mol·L- 1 的 (+ ) ,(- )一叶萩碱对基础状态下的PS无影响 ,对HFS诱导的LTP也无增强作用。将终浓度提高至 2× 10 - 8,2× 10 - 7mol·L- 1 时 ,两种构型一叶萩碱对基础PS和HFS诱导的LTP有明显的增强作用 ,GABA能完全拮抗此作用。结论 (+ ) ,(- )一叶萩碱在相同浓度下产生相似的效应 ,它们可能通过拮抗GABA受体参与LTP的形成和维持
Objective To observe the effects of levorotatory and dextrosiderine on the synaptic transmission function of hippocampal dentate gyrus in anesthetized rats, and further study its mechanism of action. Methods In vivo recording of synaptic transmission long-term potentiation (LTP) electrophysiological methods, recording the hippocampal dentate gyrus granule cell population peak (PS). Results The final concentrations of 2×10 -9mol·L -1 (+) and (-)-campesine had no effect on the PS in the basal state, and no effect on the HTP-induced LTP. When the final concentration was increased to 2×10 -8 and 2×10 -7 mol·L -1 , two kinds of configuration, ie, foliarine, significantly enhanced the LTP induced by basic PS and HFS, and GABA could completely antagonize this effect. . CONCLUSION: (+) and (-)-campesin have similar effects at the same concentration. They may participate in the formation and maintenance of LTP by antagonizing GABA receptors.