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目的探讨乳腺癌肿瘤血管生成与淋巴结转移的生物学行为关系。方法在９２例原发性乳腺浸润性导管癌术后病例的石蜡包埋肿瘤组织标本中，应用免疫组化方法检测血管内皮生长因子（ＶＥＧＦ）表达程度和新生血管数目，并采用目镜网格测微尺和图像分析系统分别对二者进行定量观察。结果ＶＥＧＦ表达程度与肿瘤新生血管数目之间具有极为显著的正相关关系（Ｐ＜０００１），新生血管数目随着ＶＥＧＦ表达程度的增高而增加。乳腺癌的淋巴结转移与ＶＥＧＦ表达程度、新生血管数目密切相关，淋巴结转移组的ＶＥＧＦ表达程度及新生血管数目明显高于淋巴结无转移组（Ｐ＜０００５～０００１）。结论ＶＥＧＦ在原发性乳腺癌的血管生成中起着重要作用，肿瘤的血管生成促进肿瘤的淋巴结转移。
Objective To investigate the biological behavior of tumor angiogenesis and lymph node metastasis in breast cancer. Methods Immunohistochemistry was used to detect the expression of vascular endothelial growth factor (VEGF) and neovascularization in paraffin-embedded tumor specimens of 92 patients with primary breast invasive ductal carcinoma. The microscopic and image analysis systems were used to quantitatively observe both. Results There was an extremely significant positive correlation between the degree of VEGF expression and the number of tumor neovascularization (P < 0.001), and the number of neovascularization increased with the increase of VEGF expression. The lymph node metastasis of breast cancer was closely related to the degree of VEGF expression and the number of new blood vessels. The degree of VEGF expression and the number of new blood vessels in the lymph node metastasis group were significantly higher than those in the no lymph node metastasis group (P<0005- 0001). Conclusion VEGF plays an important role in the angiogenesis of primary breast cancer. Tumor angiogenesis promotes lymph node metastasis.