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[Objective]To explore the methods for extraction and content determination of polysaccharides from Hedysayum polybotrys Hand.-Mazz.,and to observe its anti-inflammatory effect. [Methods] Polysaccharides were extracted by hot water. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chromomeric agent. [Results]The content of HPS was 4. 94% in same batch of radix hedysari slices,while that reached 45. 30% in refined radix hedysari samples. HPS inhibited the xylene-induced ear swelling in mice.[Conclusions]The phenol-sulfuric acid method is simple,rapid,and accurate,and HPS has anti-inflammatory effect.
[Objective] To explore the methods for extraction and content determination of polysaccharides from Hedysayum polybotrys Hand-Mazz., And to observe its anti-inflammatory effect. [Methods] Polysaccharides were extracted by hot water. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chromomeric agent. [Results] The content of HPS was 4. 94% in the same batch of radix hedysari slices, while that reached 45. 30% in refined radix hedysari samples. HPS inhibited the xylene-induced ear swelling in mice. [Conclusions] The phenol-sulfuric acid method is simple, rapid, and accurate, and HPS has anti-inflammatory effect.