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目的：克服亲和层析纯化抗人绒毛膜促性腺激素抗体（抗ｈＣＧ抗体）ＩｇＧ中载体与配体联接时不良的空间效应，以提高亲和层析的效率。方法：用６－氨基己酸为手臂通过溴化氰连接到Ｓｅｐｈａｒｏｓｅ４Ｂ上形成一种新的层析介质，然后将ｈＣＧ与之偶联用以纯化抗ｈＣＧ的抗体ＩｇＧ。结果：用该种亲和层析介质纯化的抗ｈＣＧ抗体ＩｇＧ回收率及免疫滴度均高于无手臂的亲和层析介质（活性回收率平均增加２２．５％，比活性平均增加５５．６％）。结论：用接手臂层析介质纯化抗ｈＣＧ抗体ＩｇＧ是一种比较好的方法。
OBJECTIVE: To overcome the adverse spatial effect of affinity chromatography of carrier and ligand in affinity chromatography purified anti-human chorionic gonadotropin (IgG) antibody IgG to improve the efficiency of affinity chromatography. METHODS: A new chromatographic medium was formed by attaching 6-aminocaproic acid as an arm to cyano-cyanogen bromide via cyanogen bromide and then coupled with hCG to purify IgG against hCG. Results: The IgG recoveries and immunoteric titres of anti-hCG antibodies purified by this affinity chromatography medium were higher than those without arm affinity chromatography medium (the average activity recovery rate increased by 22.5% and the specific activity increased by 55%). 6%). Conclusion: It is a good method to purify anti-hCG antibody IgG using arm chromatography medium.