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目的:研究两种迷迭香脂溶性提取物对MCAO型大鼠脑缺血的影响。方法:采用大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,观察与比较迷迭香的乙酸乙酯提取物和三氯甲烷提取物对MCAO型大鼠脑缺血的保护作用。结果:以20、40、80 mg/kg的剂量连续灌胃5天,乙酸乙酯提取物20 mg/kg组和三氯甲烷提取物80 mg/kg组能明显改善模型大鼠的神经功能障碍;乙酸乙酯提取物20 mg/kg组和三氯甲烷提取物40、80 mg/kg组能明显减轻模型大鼠的脑梗塞范围,对脑系数有降低趋势;乙酸乙酯提取物三个剂量组和三氯甲烷提取物80 mg/kg组能明显降低模型大鼠ADP诱导的血小板聚集率,三氯甲烷提取物80 mg/kg组还能明显降低模型大鼠FIB的含量,乙酸乙酯提取物的20、40 mg/kg剂量组和三氯甲烷提取物三个剂量组均能不同程度降低造模大鼠LDH的释放,其中以三氯甲烷提取物80 mg/kg组作用较为明显。结论:迷迭香脂溶性提取物对MCAO型大鼠脑缺血有显著保护作用。
Objective: To study the effects of two rosemary fat-soluble extracts on cerebral ischemia in MCAO rats. Methods: The middle cerebral artery occlusion (MCAO) model of rats was used to observe and compare the protective effects of ethyl acetate extract and trichloromethane extract of rosemary on cerebral ischemia in MCAO rats. Results: After continuous gavage for 20 days at doses of 20, 40 and 80 mg / kg, ethyl acetate extract at 20 mg / kg and chloroform extract at 80 mg / kg significantly improved neurological dysfunction in model rats ; Ethyl acetate extract 20 mg / kg group and chloroform extract 40,80 mg / kg group can significantly reduce the cerebral infarction range in model rats, the brain coefficient decreased; three ethyl acetate extract dose Group and chloroform extract 80 mg / kg group can significantly reduce ADP induced platelet aggregation rate in rats, chloroform extract 80 mg / kg group can significantly reduce the content of FIB model rats, ethyl acetate extraction The dosage of 20,40 mg / kg and chloroform extract of three groups could all reduce the release of LDH in the model rats to different extents, especially in the chloroform extract 80 mg / kg group. Conclusion: Rosmarinus officinalis fat-soluble extract has a significant protective effect on cerebral ischemia in MCAO rats.