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目的研究小分子干扰RNA(siRNA)逆转胃癌多药耐药细胞亚系SGC7901/VCR mdr1介导的多药耐药效应。方法根据mdr1cDNA已知序列,设计并体外转录2条含21个核苷酸的siRNA(mdr1si2631和mdr1si3071),转染SGC7901/VCR细胞,用RT-PCR检测mdr1基因mRNA的表达,免疫组织化学检测P-gp的表达,流式细胞仪检测阿霉素在细胞内的蓄积,MTT法检测细胞对阿霉素的敏感性。结果siRNA转染SGC7901/VCR细胞48 h后,mdr1基因mRNA和P-gp的表达水平下降,细胞内阿霉素积累量增加,对阿霉素敏感性的相对逆转率各达79.59%及59.98%。结论siRNA可逆转SGC7901/VCR细胞mdr1介导的多药耐药。
Objective To investigate the effect of small interfering RNA (siRNA) on multidrug resistance induced by mdr1 in gastric cancer multidrug resistance cell line SGC7901 / VCR. Methods Two siRNAs with 21 nucleotides (mdr1si2631 and mdr1si3071) were designed and in vitro transcribed into SGC7901 / VCR cells according to the known sequence of mdr1 cDNA. The expression of mdr1 mRNA was detected by RT-PCR. The expression of P -gp expression, flow cytometry adriamycin in the accumulation of cells, MTT assay cells to doxorubicin sensitivity. Results After siRNA transfected SGC7901 / VCR cells for 48 h, the expression levels of mdr1 mRNA and P-gp decreased, the intracellular adriamycin accumulation increased, and the relative reversal rates of doxorubicin sensitivity reached 79.59% and 59.98% . Conclusion siRNA can reverse mdr1-mediated multidrug resistance in SGC7901 / VCR cells.