【摘 要】
:
While host proteins incorporated into virions during viral budding from infected cell are known to play essential roles in multiple process of the life cycle of progeny virus,these characteristics have been largely neglected in studies on rabies virus (RA
【机 构】
:
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterina
论文部分内容阅读
While host proteins incorporated into virions during viral budding from infected cell are known to play essential roles in multiple process of the life cycle of progeny virus,these characteristics have been largely neglected in studies on rabies virus (RABV).Here,we purified the RABV virions with good purity and integrity,and analyzed their proteome by nano LC-MS/MS,followed by the confirmation with immunoblot and immuno-electronic microscopy.In addition to the 5 viral proteins,49 cellular proteins were reproducibly identified to be incorporated into matured RABV virions.Function annotation suggested that 24 of them were likely involved in virus replication.Furthermore,cryo-EM was employed to observe the purified RABV virions,generating high-resolution pictures of the bullet-shaped virion structure of RABV.This study has provided new insights into the host proteins composition in RABV virion and shed the light for further investigation on molecular mechanisms of RABV infection,as well as the discovery of new anti-RABV therapeutics.
其他文献
Influenza is one of the oldest infectious diseases affecting humans.Every influenza pandemic in history has ended with disastrous outcomes regarding public health and the social economy.This year marks the 100th anniversary of the Spanish flu (H1N1) outbr
Innate immunity provides immediate defense against viral infection.Influenza A virus (IAV) is able to get past the first line of defense.Elucidation of the molecular interaction between influenza factors and the newly recognized host players in the innate
Chikungunya virus (CHIKV) is an arbovirus transmitted by Aedes mosquitos in tropical and subtropical regions across the world.After decades of sporadic outbreaks,it re-emerged in Africa,Asia,India Ocean and America suddenly,causing major regional epidemic
Epstein-Barr virus (EBV) is an important human dsDNA virus,which has been shown to be associated with several malignancies including about 10% of gastric carcinomas.How EBV enters an epithelial cell has been an interesting project for investigation.“Cell-
Ticks are important vectors for the transmission of pathogens including viruses.The viruses carried by ticks also known as tick-borne viruses (TBVs),contain a large group of viruses with diverse genetic properties and are concluded in two orders,nine fami
As specific pathogens of noctuid pests,including Spodoptera exigua,S.litura,Helicoverpa armigera,and Mythimna separata,ascoviruses are suitable for the development of bioinsecticides.In this study,the infectivity of Heliothis virescens ascovirus 3j (HvAV-
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic tick-borne bunyavirus that causes lethal infectious disease and severe fever with thrombocytopenia syndrome (SFTS) in humans.The molecular mechanisms and host cellular factor