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目的:探讨疑难血型鉴定的快速分析判断路径。方法:通过305例疑难血型送检标本鉴定的回顾性分析,了解扬州地区疑难血型及不规则抗体分布,分析红细胞抗原及血清抗体的增减改变,建立疑难血型分析思路。结果:疑难血型中,血型抗原以A亚型为主,抗体以冷凝集素及Rh血型系统抗体为主。结论:排除标本差错与污染,正反定型结果不一致时,选择性进行吸收放散试验,检测不规则抗体,进行唾液血型物质检测和Rh表型分析等能有效解决疑难血型的鉴定。
Objective: To explore the rapid analysis of difficult blood type identification path. Methods: A retrospective analysis of 305 samples with difficult blood type was conducted to find out the distribution of difficult blood type and irregular antibody in Yangzhou area. The changes of erythrocyte antigen and serum antibody were analyzed, and the idea of difficult blood type analysis was established. Results: In the difficult blood group, the blood group antigen was mainly A subtype, and the antibody was mainly cold agglutinin and Rh blood group antibody. CONCLUSIONS: When the errors and contamination of specimens are excluded and the results of positive and negative stereotypes are inconsistent, the selective absorption and desorption tests, the detection of irregular antibodies, the detection of saliva blood substances and the analysis of Rh phenotype can effectively resolve the identification of difficult blood types.