论文部分内容阅读
观察人T细胞对全身型重症肌无力 (MG )患者抗乙酰胆碱受体抗体 (AchR Ab )产生的影响。在体外用乙酰胆碱受体(AchR )为抗原诱导 30例全身型MG患者的淋巴细胞 ,并分别加入正常人T细胞和同种异体MG患者T细胞共同培养后 ,用酶联免疫吸附实验 (ELISA )检测MG患者淋巴细胞培养上清中AchR Ab的含量 ,并用微量细胞毒试验检测培养前后MG患者的T细胞亚群。结果显示 ,加入正常人T细胞组 ,其AchR Ab含量显著低于加入同种异体MG患者T细胞组和MG患者自身对照组 ,且正常人T细胞可使MG患者T细胞亚群间的比例发生改变 ,CD8+细胞数量明显增多 ,CD4+/CD8+比值明显降低。本文提示正常人T细胞能抑制全身型MG患者AchR Ab的产生 ,并对可能的机制及潜在的应用意义作了讨论。
To observe the effect of human T cells on the production of anti-acetylcholine receptor antibody (AchR Ab) in patients with systemic myasthenia gravis (MG). T lymphocytes from 30 patients with systemic MG were induced by AchR antigen in vitro and were added into T cells of normal human T cells and allogeneic MG patients respectively. After co-cultured with T cells, enzyme linked immunosorbent assay (ELISA) The content of AchR Ab in the lymphocyte culture supernatant of MG patients was detected, and the T cell subsets of MG patients before and after culture were detected by using the cytotoxicity assay. The results showed that adding normal T cell group, the AchR Ab content was significantly lower than the addition of allogenic MG patients with T cells and MG patients with self-control group, and normal T cells MG patients can make the proportion of T-cell subsets Change, the number of CD8 + cells increased significantly, CD4 / CD8 ratio was significantly reduced. This article suggests that normal human T cells can inhibit the production of AchR Ab in patients with systemic MG, and the possible mechanisms and potential applications are discussed.