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目的完善溪黄草的定性定量方法,对药品质量进行有效控制。方法采用薄层鉴别法,对溪黄草中迷迭香酸、齐墩果酸和芦丁进行鉴别。采用高效液相色谱(HPLC法)测定迷迭香酸的含量,以十八烷基硅烷键合硅胶为填充剂;以乙腈-0.1%磷酸溶液(30∶70)为流动相;流速为1.0 mL·min-1;检测波长为330 nm;柱温为25℃;理论塔板数按迷迭香酸峰计算不低于4000。结果 TLC斑点清晰,分离度高,方法耐用性好;HPLC方法简便、专属性强,迷迭香酸线性范围为1.2μg~12μg(r=0.9999),平均回收率为98.37%,RSD为1.20%(n=6)。结论所建立的定性、定量方法可用于溪黄草的质量控制。
Objective To improve the qualitative and quantitative method of Xihuangcao, and to control the quality of the medicine effectively. Methods The TLC method was used to identify rosmarinic acid, oleanolic acid and rutin in Xihuangcao. The content of rosmarinic acid was determined by high performance liquid chromatography (HPLC). The octadecylsilane bonded silica was used as the filler. The mobile phase was acetonitrile-0.1% phosphoric acid solution (30:70) · Min-1; detection wavelength is 330 nm; column temperature is 25 ℃; the number of theoretical plates is calculated as rosmarinic acid peak not less than 4000. Results The HPLC method was simple and specific. The linear range of rosmarinic acid was 1.2μg ~ 12μg (r = 0.9999), the average recovery was 98.37% and the RSD was 1.20% (n = 6). Conclusion The established qualitative and quantitative methods can be used for the quality control of Xihuangcao.