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姐妹染色单体互换(SCE)是各种化学因子引起DNA 损伤的敏感指标。由于某些化学因子在离体条件下未见诱变作用,而在活体条件下却能诱发一定量的姐妹染色单体互换,因此体内SCE 的研究在诱变剂和致癌剂的筛选中日益得到重视。体内SCE 研究最大困难是BudR 在宿主动物体内迅速而大量降解,因而给BudR 充分标记染色体带来了极大的困难。为解决这一困难,目前多采用BudR对宿主动物连续注射一个细胞周期(每小时一次)或者连续静脉注射二个细胞周期。或用BudR 片剂皮下埋植缓慢释放,为体内细胞增殖提供足够的Bu-dR。1979年Kanda 等首先报道了观察体内SCE 的
Sister chromatid exchange (SCE) is a sensitive indicator of DNA damage caused by various chemical agents. Because some chemical factors have not been mutagenized in vitro, but can induce a certain amount of sister chromatid exchange in living conditions, the study of in vivo SCE is increasingly used in the screening of mutagens and carcinogens Receive attention. One of the biggest difficulties with in vivo SCE research is the rapid and extensive degradation of BudR in host animals, which has caused great difficulties for BudR to fully map chromosomes. In order to solve this problem, the host animal is currently using BudR to continuously inject one cell cycle (once per hour) or two consecutive cell cycles of intravenous injection. Or slow release implanted subcutaneously in BudR tablets, providing sufficient Bu-dR for cell proliferation in vivo. In 1979 Kanda et al. First reported the observation of in vivo SCE