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糖槭为高大落叶乔木,是“先割糖,后用材”的理想经济树种,现北美已广泛种植,我国不少地方亦在引种栽培。但目前糖槭苗甚缺。为探索一种新的快速繁殖方法,我们从1978年开始,试用糖槭茎尖进行离体培养,经过反复试验,已初步获得一批完整植株。试验方法及结果如下: 1.所用材料为糖槭扦插苗。取其枝条顶端部分,长约2—3厘米,用水洗净,在无菌条件下,将枝条置于70％酒精中浸泡10—15秒钟后用饱和漂白粉清液浸泡消毒15—20分钟,再用无菌水冲洗2—3次,然后剥取0.2—0.4厘米的茎尖接种在培养基上。每管接种1—2个。
Sugar maple as tall deciduous trees, is “the first cut sugar, timber after use,” the ideal economic species, is now North America has been widely planted in many parts of our country is also the introduction of cultivation. However, the current shortage of sugar maple seedlings. In order to explore a new method of rapid propagation, we started from the beginning of 1978 and experimented on the tips of sugar-black maple to culture in vitro. After repeated experiments, a number of complete plants were initially obtained. Test methods and results are as follows: 1. The material used is sugar maple cuttings. Take the top part of its branches, about 2-3 cm, washed with water, under sterile conditions, the branches placed in 70% alcohol soak 10-15 seconds after soaked in saturated bleach serum disinfection 15-20 minutes, Then rinse 2-3 times with sterile water and then stripped 0.2-0.4 cm shoot tips on medium. Inoculation 1-2 per tube.