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目的:观察赛特铂对人卵巢癌细胞A2780的凋亡诱导作用及对细胞周期的影响。方法:MTT法观察药物对细胞增殖的抑制作用,碘化丙锭染色分析细胞周期变化,电镜和荧光显微镜观察细胞形态学变化,多参数流式细胞术及TUNEL法检测细胞凋亡率,并测定caspase-3的活性变化及caspase抑制剂对细胞存活率的影响。结果:赛特铂可抑制A2780细胞的增殖并诱导凋亡,具有明显的剂量依赖性,作用与顺铂相当。赛特铂主要导致A2780细胞S期细胞明显增加,G2/M期细胞少许增加。经赛特铂作用后漂浮细胞呈现典型凋亡细胞形态学改变。caspase-3活性随着细胞凋亡率增加而增加,caspase抑制剂不完全抑制细胞死亡。结论:赛特铂影响A2780细胞周期分布,可体外诱导A2780细胞凋亡。caspase依赖性和caspase非依赖性途径参与了其凋亡过程,其中caspase依赖性途径又包括caspase-3依赖性和非caspase-3依赖性途径。
Objective: To observe the apoptosis-inducing effect of satraplatin on human ovarian cancer cell A2780 and its effect on cell cycle. METHODS: MTT assay was used to observe the inhibitory effects of drugs on cell proliferation. Propidium iodide staining was used to analyze the changes of cell cycle. Electron microscopy and fluorescence microscopy were used to observe the changes of cell morphology. Multi-parameter flow cytometry and TUNEL assay were used to detect the apoptosis rate. Changes in caspase-3 activity and the effect of caspase inhibitors on cell viability. RESULTS: Sateplatin inhibited the proliferation of A2780 cells and induced apoptosis in a dose-dependent manner. The effect was similar to that of cisplatin. Sateplatin caused a significant increase in S phase cells in A2780 cells, and a slight increase in G2/M phase cells. After being treated with Seteplatin, floating cells showed typical apoptotic cell morphological changes. The activity of caspase-3 increases with increasing apoptosis rate, and caspase inhibitors do not completely inhibit cell death. CONCLUSION: Sateplatin affects the cell cycle distribution of A2780 and induces apoptosis of A2780 cells in vitro. The caspase-dependent and caspase-independent pathways are involved in the process of apoptosis. The caspase-dependent pathways include caspase-3-dependent and non-caspase-3-dependent pathways.