已知DNA或核酸免疫能在体内诱导抗原特异性的体液和细胞免疫,而且应用分子佐剂如共刺激分子、细胞因子等可增强和调节这种免疫反应。本实验旨在进一步研究分子佐剂在机体免疫应答中的作用。为此,作者构建了表达人类免疫缺陷病毒1型(HIV-1)和猴免疫缺陷病毒(SIV)gag/pol蛋白的DNA疫苗,并分别将编码Th1细胞因子IL-2、IL-12,Th-2细胞因子IL-4、IL-10和GM-CSF基因克隆到pcDNA3表达载体中,体外转染证实上述细胞因子可正常表达。然而,将 DNA or nucleic acid immunization is known to induce antigen-specific humoral and cellular immunity in vivo, and the use of molecular adjuvants such as costimulatory molecules, cytokines and the like can enhance and modulate this immune response. This experiment aims to further study the role of molecular adjuvants in the immune response in the body. To this end, the authors constructed a DNA vaccine that expresses the human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) gag / pol proteins and respectively encoded the Th1 cytokines IL-2, IL- 12, Th -2 cytokines IL-4, IL-10 and GM-CSF genes were cloned into pcDNA3 expression vector, transfected in vitro confirmed that the above cytokines can be normal expression. However, will