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目的 探讨环磷酰胺对荷瘤鼠HAC肝癌细胞凋亡和增殖的影响。方法 建立小鼠HAC肝癌荷瘤模型 ,用TUNEL法和免疫组化法检测环磷酰胺作用下 ,HAC肝癌细胞的凋亡和PCNA表达的变化。结果 15mg kg环磷酰胺的抑瘤率 43 %。HAC肝癌细胞的凋亡指数 1 93 % ,较荷瘤对照组 1 11%明显升高 (P <0 0 5 )。增殖指数 35 75% ,较荷瘤对照组 5 6 38%明显下降 (P <0 0 1)。结论 环磷酰胺有诱导HAC肝癌细胞凋亡和抑制细胞增殖的作用
Objective To investigate the effect of cyclophosphamide on apoptosis and proliferation of tumor-bearing mice with HAC. Methods The mouse model of HAC liver cancer was established. The apoptosis and PCNA expression of HAC hepatoma cells were detected by TUNEL and immunohistochemistry. Results The inhibitory rate of 15 mg cyclophosphamide was 43%. The apoptosis index of HAC hepatoma cells was 93%, which was significantly higher than that of the tumor-bearing control group (11%) (P <0 05). The proliferation index was 35 75%, which was significantly lower than that of the tumor-bearing control group (536) (P < 0.01). Conclusion Cyclophosphamide can induce apoptosis of hepatocellular carcinoma cells and inhibit cell proliferation.