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对于角膜缘干细胞(LSCs)缺乏导致的眼表疾病,角膜移植是较好的治疗方法,传统角膜移植术的开展受供体来源匮乏、术后排斥反应等因素的影响.构建组织工程角膜上皮进行移植很大程度上解决了供体来源和术后免疫排斥的问题,成为重要和有效的治疗方法,而种子细胞的选择是手术成功的关键.随着组织工程技术和细胞培养技术的不断发展,组织工程角膜种子细胞方面的研究越来越多,种子细胞的可选择性也越来越大,如胚胎干细胞(ESCs)、LSCs等研究已经逐步展开.就目前体外重建组织工程角膜上皮研究中使用的种子细胞及其近年来的相关研究进展进行综述.“,”Keratoplasty is a choice for the treatment of ocular surface diseases caused by corneal limbal stem cells (LSCs) deficiency.The application of traditional keratoplasty is limited by avaibility of donor corneas and allograft rejection.Constructruction of tissue engineering corneal epithelium provides an important and effective approach to the transplantation of cornea,because it can solve the lack of donor corneas and avoid allograft rejection following keratoplasty.However,the selection of the seed cells is crucial to corneal tissue engineering.What is more,the research of seed cells is becoming more and more widespread,just like embryonic stem cells (ESCs) and LSCs.This article summarized the selection of seed cells and the progress of tissue-engineered human corneal epithelium.