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【目的】探讨清热除痹汤含药血清对尿酸钠结晶刺激下THP-1细胞的增殖活性及分泌白细胞介素-1β(IL-1β)功能的影响。【方法】体外培养人单核细胞THP-1细胞,分为5组,空白血清组,模型对照组,中药血清高、中、低浓度组(浓度分别为体积分数20%、10%、5%),除空白血清组外,其他各组均加入浓度为500 mg/L的尿酸钠结晶,于培养0、12、24、48 h时间点采用四甲基偶氮唑盐比色(MTS)法检测细胞的增殖活性,培养48 h后采用酶联免疫吸附(ELISA)法检测细胞上清液IL-1β含量。【结果】各组THP-1细胞活性均随着时间的延长而增加,模型对照组各时间点细胞活性均较空白血清组显著增高(P<0.05或P<0.01),48 h模型对照组的IL-1β水平较空白血清组显著增高(P<0.01)。12、24 h中药血清各浓度组,48 h中药血清高、中浓度组细胞活性均较模型对照组显著下降(P<0.05或P<0.01),48 h中药血清各浓度组IL-1β水平均较模型对照组显著下降(P<0.01)。【结论】清热除痹汤含药血清对尿酸钠结晶刺激下THP-1细胞的活性有抑制作用,机制与其可抑制IL-1分泌有关。
【Objective】 To investigate the effect of Qingre Chu Bi Decoction serum on proliferation activity and secretion of interleukin-1β (IL-1β) in THP-1 cells stimulated by sodium urate. 【Methods】 Human monocytic THP-1 cells were cultured in vitro and divided into five groups: blank serum group, model control group and Chinese medicine serum high, medium and low concentration groups (concentration: 20%, 10%, 5% ), Except for the blank serum group, the other groups were added with sodium urate crystals at the concentration of 500 mg / L. At the time point of 0, 12, 24 and 48 h, the MTS method The proliferative activity of the cells was detected. After cultured for 48 h, the content of IL-1β in the supernatant of the cells was detected by enzyme-linked immunosorbent assay (ELISA). 【Results】 The activity of THP-1 cells in each group increased with time. The cell viability in model control group was significantly higher than that in blank serum group (P <0.05 or P <0.01) IL-1β levels were significantly higher than those in blank serum group (P <0.01). The serum levels of IL-1β in serum of 48 and 24 h groups decreased significantly (P <0.05 or P <0.01) compared with the model control group at 48 and 48 h Compared with the model control group decreased significantly (P <0.01). 【Conclusion】 Qingre Chu Bi Decoction serum can inhibit the activity of THP-1 cells stimulated by sodium urate, and its mechanism may be related to the inhibition of IL-1 secretion.