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胰酶消化法培养鼠颅骨成骨细胞,并与骨髓细胞建立OB-OC共培养体系;体系中加入10-6mol/LPGE2、10-8mol/L VitD3诱导破骨细胞分化生成。实验分对照组和ALN(10-6mol/L)处理组。采用TRAP染色及扫描电镜检测破骨
The pancreatic osteoblasts were cultured with trypsin digestion, and OB-OC co-culture system was established with bone marrow cells. Osteoclast differentiation was induced by the addition of 10-6mol / LPGE2,10-8mol / L VitD3 in the system. Experimental points control group and ALN (10-6mol / L) treatment group. Using TRAP staining and scanning electron microscopy to detect osteoclasts