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目的建立甲氰菊酯犬灌胃染毒致死模型并改进了生物检材中甲氰菊酯的气相色谱和气相色谱-质谱联用检测方法,研究甲氰菊酯在犬体内的死后分布规律。方法犬6只以8LD50(5.2 g/kg)剂量甲氰菊酯经口灌胃染毒死亡后,分别取心、肝、脾、肺、肾、脑、胃、胸肌、右后肢肌、心血、尿、胆汁、玻璃体液等脏器组织和体液,GC和GC/MS检测其甲氰菊酯含量。结果犬8LD50甲氰菊酯灌胃染毒后在1.45～2.05 h出现中毒症状,染毒后4.5～7.8 h死亡。气相色谱和气相色谱-质谱联用法除胆汁外均检测到甲氰菊酯,甲氰菊酯在中毒死亡犬体内分布趋势为:胃壁(2.196±1.229)μg/g、心血(0.650±0.237)μg/ml、肝(0.560±0.260)μg/g、心(0.318±0.256)μg/g、尿液(0.275±0.202)μg/ml、肺(0.201±0.121)μg/g、脑(0.193±0.103)μg/g、肾(0.188±0.117)μg/g、脾(0.183±0.050)μg/g、胸肌(0.149±0.026)μg/g、玻璃体液(0.138±0.085)μg/g、右后肢肌(0.109±0.070)μg/g,胆汁中未检出。结论甲氰菊酯的灌胃染毒致死模型、气相色谱和气相色谱/质谱联用检测方法和体内死后分布规律可应用于其法医毒物动力学研究和中毒致死案件的法医学鉴定。
OBJECTIVE To establish a model of perinatal exposure to fenpropathrin in dogs and improve the detection of fenpropathrin in the samples by gas chromatography and gas chromatography-mass spectrometry (GC-MS) . Methods Six dogs were dosed with 8LD50 (5.2 g / kg) dose of fenpropathrin to induce cardiac dysfunction in rats with heart, liver, spleen, lung, kidney, brain, stomach, Urine, bile, vitreous humor and other organs and body fluids, GC and GC / MS detection of fenpropathrin content. Results The symptoms of poisoning were observed in 1.45 ~ 2.05 h after inoculation with 8D50 fenpropathrin in dogs, and 4.5 ~ 7.8 h after exposure. The results showed that fenpropathrin and fenpropathrin were detected in the dogs died of poisoning by gas chromatography and gas chromatography-mass spectrometry (GC-MS), respectively. The distribution trends of fenpropathrin in drowned dogs were gastric wall (2.196 ± 1.229) μg / g and blood (0.650 ± 0.237) μg (0.218 ± 0.256) μg / g, 0.275 ± 0.202 μg / ml and 0.201 ± 0.121 μg / g in the liver and 0.193 ± 0.103 in the brain, (0.188 ± 0.117) μg / g, spleen (0.183 ± 0.050) μg / g, breast muscle (0.149 ± 0.026) μg / g, vitreous humor (0.138 ± 0.085) μg / g, ± 0.070) μg / g, not detected in bile. Conclusion The gavage model of fenpropathrin induced by gavage can be applied to the forensic identification of forensic toxicokinetic studies and poisoning death cases by gas chromatography, gas chromatography / mass spectrometry combined with postmortem distribution.