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以紫丁香、皂角为材料研究亚硫酸对叶片膜质过氧化的影响,结果表明,低浓度(5mmol/L)的NaHSO3,使叶片的SOD活性上升,MDA含量下降。高于5mmol/L的NaHSO3使SOD活性下降,MDA含量升高;且NaHSO3浓度越大,SOD、MDA变化幅度越大,NaHSO3处理也使得叶绿素———蛋白质结合度下降。植物材料经磷酸缓冲液喷洒后,膜质过氧化程度降低,表现为在各种浓度NaHSO3处理中,叶片的SOD活性、叶绿素———蛋白质结合度均高于未喷磷液者;而MDA含量均低于未喷者。表明磷液对亚硫酸伤害具有防护作用,防护效果与植物材料、亚硫酸浓度和生理指标有关。
Using lilac and saponin as materials, the effects of sulfite on the membrane lipid peroxidation were studied. The results showed that NaHSO3 at low concentration (5 mmol / L) increased SOD activity and decreased MDA content. When NaHSO3 was above 5mmol / L, SOD activity decreased and MDA content increased. With the increase of NaHSO3 concentration, the changes of SOD and MDA increased. NaHSO3 also decreased the chlorophyll-protein binding. When the plant material was sprayed with phosphate buffer, the degree of membrance peroxidation decreased. The results showed that SOD activity and chlorophyll-protein binding of leaves were higher than that of non-phosphated solution in various concentrations of NaHSO3 treatment. However, MDA content All were lower than those who did not spray. The results showed that the phosphorus solution had a protective effect on the sulfitation injury, and the protective effect was related to the plant material, the sulfite concentration and the physiological index.