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以转基因抗虫棉Gossypium hirsutum L.中棉所41、中棉所45和新棉99B为试材,通过水培试验研究了冠菌素对盐胁迫条件下棉花幼苗中渗透调节物质和激素水平的影响。结果表明:在盐胁迫条件下,冠菌素可提高棉花中脯氨酸含量(冠菌素处理后脯氨酸的含量是用盐处理的2.1倍),0.01、0.1和1μmol/L的冠菌素处理分别使棉花真叶中脱落酸(ABA)含量提高27%、119%和239%,0.1和1μmol/L的冠菌素处理使棉花根系中的ABA含量分别提高222%和130%;冠菌素也可提高棉花中茉莉酸类物质(JAs)和真叶中吲哚-3-乙酸(IAA)的含量,诱导与盐胁迫相关蛋白的表达,保护细胞膜的稳定性,增强盐胁迫下棉花体内的渗透调节能力。
Using transgenic cotton Gossypium hirsutum L. Zhongmiansuo 41, Zhongmiansuo 45 and Xinmian 99B as test materials, the effects of coronatine on osmotic adjustment substances and hormone levels in cotton seedlings under salt stress influences. The results showed that under salt stress, coronatine could increase proline content in cotton (2.1 times proline content after treatment with coronatine), 0.01, 0.1 and 1μmol / L crown bacteria The abscisic acid (ABA) content in cotton leaves increased by 27%, 119% and 239%, respectively. The levels of ABA in cotton roots increased by 222% and 130% respectively when treated with 0.1 and 1μmol / Mycin also increased the contents of jasmonic acid (JAs) and indole-3-acetic acid (IAA) in true leaves, induced the expression of proteins related to salt stress, protected the stability of cell membrane and enhanced the activity of cotton under salt stress Osmotic regulation in the body.