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目的:建立高效液相色谱法测定清空软胶囊中芍药苷含量的方法。方法:采用Diamonsil C18(5μm,4.6×200mm)色谱柱,以甲醇-水-冰醋酸(25:75:0.5)为流动相,流速1.2 mL/min,检测波长230 nm。结果:在给定的色谱条件下,芍药苷与相邻组份色谱峰分离度良好,芍药苷在0.1004～2.008μg范围内线性关系良好,r=0.9998,平均回收率为98.5%。结论:所建立的方法操作简便,结果准确,重现性好,可以控制清空软胶囊的质量。
Objective: To establish a method for determination of paeoniflorin in soft capsules by high performance liquid chromatography. Methods: Diamonsil C18 (5μm, 4.6 × 200mm) column was used with mobile phase of methanol - water - acetic acid (25:75:55) at a flow rate of 1.2 mL / min and a detection wavelength of 230 nm. Results: The chromatographic peaks of paeoniflorin and its adjacent components were well separated under the given chromatographic conditions. Paeoniflorin had a good linearity in the range of 0.1004 ~ 2.008 μg with the average recovery of 98.5%. Conclusion: The established method is simple, accurate, reproducible and can control the quality of soft capsules.