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目的了解来自于江西地区胃十二指肠疾病儿童感染的幽门螺杆菌(H.pylori)临床分离菌株cag A、vac A和ice A基因亚型分布情况,并探讨H.pylori cag A、vac A和ice A基因亚型与儿童胃十二指肠疾病类型之间的关系。方法从来自江西地区的316例患有胃十二指肠疾病儿童的胃窦黏膜中,培养出107株H.pylori菌株,提取菌株基因组DNA,采用PCR法检测H.pylori ure A、cag A、vac A及ice A基因亚型。结果在107株H.pylori临床分离菌株中,H.pylori ure A基因和cag A基因检出率分别为100%(107/107)和94.4%(101/107)。vac A基因总检出率为100%(107/107),vac As1a、vac As1c、vac Am1和vac Am2基因检出率分别为74.8%(80/107)、25.2%(27/107)、29.9%(32/107)和69.2%(74/107),其中0.9%(1/107)H.pylori菌株同时检测出vac Am1和vac Am2基因型;在vac A基因的嵌合体中,vac As1a/m1、vac As1a/m2、vac As1c/m1和vac As1c/m2基因检出率分别为26.2%(28/107)、51.4%(55/107)、3.7%(4/107)和17.8%(19/107)(P<0.001)。ice A1和ice A2基因阳性检出率分别为79.4%(85/107)和9.3%(10/107)(P<0.001);ice A1和ice A2基因亚型均阳性的检出率为7.5%(8/107)。H.pylori各基因亚型在消化性溃疡、慢性胃炎和十二指肠球炎3组间的检出率比较差异无统计学意义(P>0.05)。结论来自于江西地区胃十二指肠疾病儿童感染的H.pylori优势基因亚型是cag A、vac As1a/m2和ice A1;H.pylori感染存在不同基因型菌株混合感染;H.pylori基因亚型与胃十二指肠疾病类型无相关性。
Objective To investigate the distribution of cag A, vac A and ice A gene subtypes in H.pylori isolates from children with gastroduodenal diseases in Jiangxi province and to investigate the relationship between H.pylori cag A, vac A And ice A gene subtypes and gastroduodenal disease in children. Methods A total of 107 strains of H.pylori were isolated from the gastric mucosa of 316 children with gastroduodenal diseases from Jiangxi province. Genomic DNA was extracted from the antral mucosa of children with gastroduodenal disease. The detection of H.pylori ure A, cag A, vac A and ice A gene subtypes. Results The positive rates of H.pylori ure A gene and cag A gene in 107 isolates of H.pylori were 100% (107/107) and 94.4% (101/107), respectively. The detection rates of vac As1a, vac As1c, vac Am1 and vac Am2 genes were 74.8% (80/107), 25.2% (27/107), 29.9 % (32/107) and 69.2% (74/107), respectively, of which 0.9% (1/107) H. pylori strains were detected simultaneously with vac Am1 and vac Am2 genotypes; in vac A gene chimeras, vac As1a / The detection rates of m1, vac As1a / m2, vac As1c / m1 and vac As1c / m2 were 26.2% (28/107), 51.4% (55/107), 3.7% (4/107) and 17.8% /107)(P<0.001). The positive detection rates of ice A1 and ice A2 genes were 79.4% (85/107) and 9.3% (10/107) (P <0.001), respectively. The positive rates of both ice A1 and ice A2 subtypes were 7.5% (8/107). There was no significant difference in the detection rate of H.pylori among the three groups of peptic ulcer, chronic gastritis and duodenal inflammation (P> 0.05). Conclusions The predominant genotypes of H.pylori in children with gastroduodenal diseases in Jiangxi province are cag A, vac As1 a / m2 and ice A1. The genotypes of H.pylori infection are mixed with different genotypes. Type and gastroduodenal disease type no correlation.