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目的:通过检测分析 ARDS 和 SIRS 病人血浆前炎症细胞因子肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、白介素6(IL-6)和抗炎细胞因子白介素10(IL-10)水平的变化,探讨这些细胞因子在全身炎症反应和急性肺损伤中的作用。方法:选择临床诊断 ARDS 病例22例和 SIRS 8例,以及正常对照10例,收集血浆,采用酶联免疫法(ELISA)检测 TNF-α、IL-1β、IL-6和 IL-10蛋白含量。结果:SIRS 病人血浆 TNF-α、IL-1β、IL-6和 IL-10含量分别为206.1±85.9 pg/ml、313.6±76.7 pg/ml、141.4±41.5 pg/ml 和259.6±54.34 pg/ml,均显著低于ARDS 病人(分别为629.3±187 pg/ml、892.4±209 pg/ml、261.1±71.3 pg/ml 和458.1±111.93 pg/ml);但SIRS 和 ARDS 病人上述细胞因子水平均显著高于正常对照组。结论:TNFα、IL-1β和 IL-6是 SIRS 和 ARDS 演变中的重要炎症细胞因子,抗炎细胞因子 IL-10的过度释放在促进炎症反应向失控发展、急性肺损伤发展成ARDS 中发挥一定作用。
OBJECTIVE: To detect the expression of plasma proinflammatory cytokines TNF-α, IL-1β, IL-6 and IL-10 in ARDS and SIRS patients.Methods: ) Levels, to explore the role of these cytokines in systemic inflammatory response and acute lung injury. Methods: Twenty-two cases of ARDS, 8 cases of SIRS and 10 cases of normal controls were selected for clinical diagnosis. The plasma concentrations of TNF-α, IL-1β, IL-6 and IL-10 were detected by enzyme linked immunosorbent assay (ELISA) Results: The plasma levels of TNF-α, IL-1β, IL-6 and IL-10 in SIRS patients were 206.1 ± 85.9 pg / ml, 313.6 ± 76.7 pg / ml, 141.4 ± 41.5 pg / ml and 259.6 ± 54.34 pg / ml Were significantly lower than ARDS patients (629.3 ± 187 pg / ml, 892.4 ± 209 pg / ml, 261.1 ± 71.3 pg / ml, and 458.1 ± 111.93 pg / ml, respectively); however, the levels of these cytokines in both SIRS and ARDS patients were significantly higher Higher than the normal control group. CONCLUSION: TNFα, IL-1β and IL-6 are important inflammatory cytokines in the development of SIRS and ARDS. The excessive release of anti-inflammatory cytokine IL-10 plays an important role in promoting the development of inflammatory response to uncontrolled development of acute lung injury into ARDS effect.