目的:评价糖化血红蛋白(HbA1c)、糖化血清蛋白、口服50 g葡萄糖筛查试验在妊娠期糖尿病诊断的临床应用价值。方法:比较正常妊娠组(234例)、糖耐量受损组(189例)、妊娠期糖尿病组(45例)的口服50 g葡萄糖筛查试验(GCT)、HbA1c、GSP检测结果。结果:3组GCT、HbA1c、GSP血清浓度比较均有统计学差异(P<0.05),阳性率;正常妊娠组GCT阳性率(17.95%)与HbA1c、GSP阳性率比较统计学差异有显著性(P<0.01),HbA1c与GSP阳性率比较,无统计学差异(P>0.05);在糖耐量受损组及妊娠期糖尿病组,GCT、HbA1c、GSP两两比较均不支持有统计学差异(P>0.05);GCT、HbA1c、GSP的方法学评价以GSP灵敏度(88.89%)最高,特异度(89.36%)最好,阴阳性预测值的价值最高。结论:糖化血红蛋白、糖化血清蛋白在GDM的诊断中有很高的临床应用价值。 Objective: To evaluate the clinical value of screening glycosylated hemoglobin (HbA1c), glycosylated serum protein, oral 50 g glucose screening test in gestational diabetes mellitus. Methods: Oral 50 g glucose screening test (GCT), HbA1c and GSP test results were compared between normal pregnancy group (234 cases), impaired glucose tolerance group (189 cases) and gestational diabetes mellitus group (45 cases). Results: The serum concentrations of GCT, HbA1c and GSP in the three groups were significantly different (P <0.05), the positive rate was higher than that in the normal pregnancy group (17.95%) and HbA1c and GSP P <0.01). There was no significant difference between HbA1c and GSP (P> 0.05). There was no significant difference in GCT, HbA1c and GSP in impaired glucose tolerance group and gestational diabetes mellitus group P> 0.05). The methodological evaluation of GCT, HbA1c and GSP showed the highest GSP sensitivity (88.89%) with the highest specificity (89.36%) and the highest negative predictive value. Conclusion: Glycated hemoglobin and glycosylated serum protein have high clinical value in the diagnosis of GDM.