采用改进酚抽法提取巴西橡胶树木质部和树皮蛋白。结果显示,在双向电泳图谱上可分辨500多个蛋白点。木质部和树皮的蛋白表达谱存在明显差异。选取48个差异蛋白点或者高丰度蛋白进行MALDI-TOF MS质谱鉴定,成功鉴定出26个蛋白,其中,与氢氰酸合成相关的两个酶是树皮特有的,而半胱氨酸转甲基酶是木质部的高丰度蛋白。 Extracting the xylem and bark proteins of Acacia pallidiflora by improved phenol extraction. The results show that in the two-dimensional electrophoresis map can distinguish more than 500 protein spots. Significant differences exist in the protein expression profiles of xylem and bark. Twenty-eight proteins were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). Of the 26 proteins identified, the two enzymes involved in the synthesis of hydrocyanic acid were bark-specific and the cysteine- Methyl enzymes are xylem high abundance proteins.