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根据甾体激素作用的经典模型,认为雌激素先结合到靶细胞的胞浆受体上,然后形成激素受体复合物并转移入核内.作者从鼠垂体瘤得到GH_3细胞株,内含有雌激素受体(kd=3.4×10~(-10)M,每个细胞的受体数=20,000～30,000)。应用细胞松弛素B使GH_3细胞脱去核,进一步密度梯度离心,得到胞质体及含有少量细胞的核质体.早期及作者研究表明:细胞松弛素B并不影响雌二醇的摄取、结合.对每个细胞的雌激素受体或受体在胞浆和核中分布亦无影响,约有75％GH,细胞可被去核。现分别测定全细胞、
According to the classic model of steroid hormone action, it is thought that estrogen first binds to the cytoplasmic receptor of target cells and then forms a hormone receptor complex and translocates into the nucleus. The authors obtained the GH_3 cell line from the mouse pituitary tumor, Hormone receptor (kd = 3.4 × 10 -10 M, receptor number per cell = 20,000 to 30,000). Application of cytochalasin B to GH_3 cells off the nucleus, further density gradient centrifugation, to obtain the cytoplasm and a small amount of cells in the cytoplasm. Early and author studies have shown that: cytochalasin B does not affect the estradiol uptake, binding There is also no effect on the distribution of estrogen receptors or receptors per cell in the cytoplasm and nucleus, about 75% of GH, and the cells can be enucleated. Are now measuring whole cells,