论文部分内容阅读
引言辐射诱发果树营养体的突变是发生在个别细胞内,由突变细胞衍生的突变组织和原组织常以突变嵌合体的形式存在。因此,分离选择突变体技术是获得突变体的关键。为了给分离选择突变技术提供依据,国外对诱发突变发生发展规律进行着研究。据报道,辐射芽长出的初生枝,低位芽比高位芽具有较高的突变频率。Lapins指出、苹果初生枝芽6～10叶节的芽携带较宽突变组织,在营养繁殖世代中出现较高的突变频率。 Campbell指出,靠近叉枝处的芽具有较高的突变频率。我们用射线处理分离具有标志基因突变性状的周缘嵌合体,研究嵌合体分离突变发生发展规律,探讨射线诱发突变发生发展规律。
Introduction Radiation induced mutations in fruit tree vegetative organisms occur in individual cells. Mutated tissues and primitive tissues derived from mutant cells often exist as mutant chimeras. Therefore, isolation of selection mutants is the key to obtaining mutants. In order to provide a basis for separation and selection of mutation technology, foreign studies on the development and regulation of induced mutation have been carried out. It has been reported that radiating buds grow in primary branches, lower buds have higher mutation frequency than higher buds. Lapins pointed out that the buds of 6-10 leaf segments of apple primary buds carry wider mutant tissues and higher frequency of mutations in the vegetative reproductive generations. Campbell pointed out that shoots near the branches have a higher frequency of mutations. We isolated the peripheral chimeras with the mutation of the marker gene by radiation, studied the development of chimerism-related mutations and explored the development of radiation-induced mutation.