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为了研究一氧化氮在血吸虫病肝损伤发病中的作用，采用Ｓ－Ｐ免疫组织化学法，观察家兔（ｎ＝４０）经皮肤感染血吸虫尾蚴后８、１２、１６、２０、２４周家兔肝诱导型一氧化氮合成酶（ｉＮＯＳ）和巨噬细胞（ＣＤ６８）的免疫组织化学表达与定位。结果显示，血吸虫病家兔肝的虫卵肉芽周围、门静脉分支管壁、肝窦壁、中央静脉壁有ｉＮＯＳ和ＣＤ６８阳性免疫反应物，肝细胞内有ｉＮＯＳ阳性免疫反应物。正常家兔肝脏ｉＮＯＳ和ＣＤ６８免疫组织化学染色均为阴性。认为一氧化氮在血吸虫肉芽形成及肝脏微循环障碍中具有重要作用
In order to investigate the role of nitric oxide in the pathogenesis of liver injury in schistosomiasis, S-P immunohistochemistry was used to observe the effect of rabbits (n = 40) Immunohistochemical expression and localization of liver-induced nitric oxide synthase (iNOS) and macrophage (CD68). The results showed that iNOS and CD68-positive immunoreactive substances around the parietal and portal vein branches, hepatic sinusoid and central venous wall of schistosomiasis rabbit liver and iNOS-positive immunoreactive substance in hepatocytes. Normal rabbit liver iNOS and CD68 immunohistochemical staining were negative. It is believed that nitric oxide plays an important role in the formation of schistosoma granulation and liver microcirculation disorder