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烯醇化酶(enolose)是糖酵解途径中提供能量的酶,它催化2-磷酸甘油酸转化为磷酸烯醇式丙酮酸。在自然界中有着广泛的分布。哺乳类动物组织中的烯醇化酶以三种亚基型存在,免疫学上定义为α、β、γ亚基,分布各异。1965年Moore使用DEAE-纤维素层析与淀粉凝胶电泳相结合的方法在神经组织中发现了二种高酸性的蛋白:14-3-2蛋白和S-100蛋白。前者即为后来认定的神经特异性烯醇化酶(NeuronSpecilic Enolase简称NSE)。因其组织特异性及与神经组织和相关肿瘤的发生、分展及
Enolose is an energetic enzyme in the glycolytic pathway that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. It has a wide distribution in nature. The enolase in mammalian tissues exists in three subtypes and is defined immunologically as α, β, and γ subunits. In 1965 Moore used DEAE-cellulose chromatography combined with starch gel electrophoresis to find two highly acidic proteins in the nervous tissue: 14-3-2 protein and S-100 protein. The former is the later identified neuron specific enolase (NSE). Because of its tissue-specificity and the occurrence and spread of nerve tissue and related tumors,