论文部分内容阅读
目的：探讨β－羧乙基锗倍半氧化物（Ｇｅ－１３２）在体内清除自由基、抗氧化作用。方法：下列药物作用于烈酒造成氧应激态的大鼠，观察其肝线粒体超氧化物歧化酶活性和肝脏的病理变化，并与维生素Ｃ进行了比较。Ｗｉｓｔａｒ大鼠随机分为４组。正常对照（Ａ），烈酒（Ｂ），烈酒＋维生素Ｃ（Ｃ），烈酒＋Ｇｅ－１３２（Ｄ）。各组大鼠常规饲喂。每种药的给药方法如下：烈酒（含体积分数为５５％乙醇），５ｇ／（ｋｇｄ），从第８天开始ｉｇ共２周；维生素Ｃ２００ｍｇ／（ｋｇｄ），从第１天开始ｓｃ共３周；Ｇｅ－１３２２００ｍｇ／（ｋｇｄ），从第１天开始ｉｇ共３周。结果：肝线粒体铜锌超氧化物歧化酶活性，烈酒组与对照组比较，其活性下降（Ｐ＜０．０５），烈酒＋Ｇｅ－１３２和烈酒＋维生素Ｃ组均与烈酒组比较，其活性有上升趋势。肝脂肪变性在烈酒＋Ｇｅ－１３２组较烈酒组为轻，烈酒＋维生素Ｃ组病变不明显。结论：Ｇｅ－１３２在一定程度上保护肝线粒体铜锌超氧化物歧化酶活性，改善肝脂肪变性，但对肝脏病变的保护作用不如高浓度维生素Ｃ。
Objective: To investigate the scavenging free radicals and anti-oxidative effects of β-carboxyethyl germanium sesquioxide (Ge-132) in vivo. Methods: The following drugs acting on spirits caused by oxygen stress in rats, observe the liver mitochondrial superoxide dismutase activity and liver pathological changes, and with vitamin C were compared. Wistar rats were randomly divided into 4 groups. Normal control (A), spirits (B), spirits + vitamin C (C), spirits + Ge-132 (D). Rats in each group were fed routinely. Each drug was administered as follows: Spirits (containing 55% ethanol by volume), 5g / (kgd), ig for 2 weeks starting on day 8; Vitamin C 200mg / (kgd) starting on day 1 A total of 3 weeks; Ge-132200mg / (kgd), ig for 3 weeks from the first day. Results: The activities of mitochondrial copper, zinc and superoxide dismutase (SOD) in the spirits group were significantly lower than those in the control group (P <0.05), and spirits + Ge-132 and spirits + vitamin C group , Its activity is on the rise. Hepatic steatosis in spirits + Ge-132 group than the spirits group was light, spirits + vitamin C group lesions were not obvious. Conclusion: Ge-132 can protect the activities of hepatic mitochondrial copper-zinc superoxide dismutase to a certain degree and improve the hepatic steatosis, but its protective effect on liver lesion is not as good as that of high-concentration vitamin C.