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种子萌发法是检测外源抗性基因在转基因植物中的表达和分析外源抗性基因在转基因植物中遗传行为的简单而直接的方法 .以转bar基因燕麦后代R2 种子为材料 ,用不同体积分数的除草剂Challenge处理种子 ,萌发的幼苗经PCR和Southerndotblot鉴定 ,发现在 0 0 3%的除草剂上萌发的幼苗 ,部分不含bar基因 ,而在0 0 7%和 0 0 9%的除草剂上萌发的幼苗 ,均含bar基因 .用 0 0 7%的除草剂测定 3个群体的转bar基因燕麦R2 代种子的萌发情况 ,结果表明 ,bar基因在R3 代的遗传符合孟德尔的单基因遗传分离规律 ,这与已报道的用PCR和Southerndotblot分析R2 代的结果相一致 .进一步的研究发现 ,用 0 0 5 %的除草剂分析上述 3个群体 ,结果与 0 0 7%的差异不显著 ;而用 0 0 9%的除草剂分析的结果与 0 0 7%的差异显著 ,后代遗传分离比偏离 3∶1.这可能是由于后代群体中bar基因的表达水平存在着个体间差异 .研究结果表明 ,0 0 5 %和 0 0 7%是种子萌发法分析燕麦中外源bar基因遗传分离规律适宜的除草剂的体积分数 .
Seed germination method is a simple and direct method to detect the expression of exogenous resistance gene in transgenic plants and to analyze the genetic behavior of the exogenous resistance gene in transgenic plants.The R2 gene of offspring of bar gene was used as material, The seeds of the fractionated Hercules Challenge were treated and germinated seedlings were identified by PCR and Southerndotblot. Seedlings germinated on 0 0 3% of herbicide were found partially free of the bar gene, whereas in the 0 0 7% and 0 0 9% The germinated seedlings all contained the bar gene.The seed germination of barley oats rodents with bar gene was determined by 0 0 7% herbicides.The results showed that the bar gene was inherited in Mendelian single The genetic segregation pattern was consistent with the reported results of PCR and Southerndotblot analysis of R2 generation.According to the further study, we found that the above 3 populations were analyzed with 0 0 5% herbicide and the difference with 0 0 7% was not While the result of 0 9% herbicide analysis was significantly different from that of 0 0 7%, and the segregation ratio of offspring deviated from 3: 1. This may be due to the presence of bar gene in offspring population Are obtained. The results showed that 005%, and 007% of seed germination Genetic analysis of isolated oat law exogenous bar suitable volume fraction of herbicides.