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植物抗病基因的克隆一直为植物遗传学家和病理学家所关注。克隆的抗病基因在氨基酸水平上表现出一定程度的相似性, 在一些区段高度保守, 如 N B S、 L R R、激酶、 L Z等。这些保守序列, 不仅有助于对抗病基因的分类及其作用机理的理解, 而且为抗病基因克隆提供了一条新途径。根据这些保守序列合成 P C R 引物, 在许多植物中已扩增出大量的抗病基因类似序列 ( R G A)。遗传作图表明 R G A 与抗病性密切相关。对 R G A 与 R 基因的关系及 R G A 的基因组分布一并进行了讨论。
Cloning of plant disease resistance genes has long been of concern to plant geneticists and pathologists. The cloned resistance gene shows a certain degree of similarity at the amino acid level, and is highly conserved in some segments, such as N B S, LR R, kinase, L Z and the like. These conserved sequences not only help to understand the classification of disease-resistant genes and their mechanism of action, but also provide a new way for cloning resistance genes. Based on these conserved sequences, the PCR primers were synthesized and a number of RGD-like sequences (R G A) have been amplified in many plants. Genetic mapping showed that R G A is closely related to disease resistance. The relationship between R G A and R genes and the genomic distribution of R G A are also discussed.