【摘 要】
：
AIM: To investigate the effect of vasoactive intestinal peptide (VIP) on pulmonary surfactants (PS) phospholipid synthesis in cultured lung explants. METHODS: L
【机 构】
：
Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, Chi
论文部分内容阅读
AIM: To investigate the effect of vasoactive intestinal peptide (VIP) on pulmonary surfactants (PS) phospholipid synthesis in cultured lung explants. METHODS: Lung explants were cultured with serum-free medium, [methyl-3H]choline incorporation, total phospholipid, phosphatidylcholine, activity of choline-phosphate cytidylyltransferase (CCT) and CCTα mRNA level in lung explants were determined. RESULTS: (1) VIP (10-10-10-7 mol/L) for 16 h promoted [methyl-3H]choline incorporation in dose dependence and VIP (10-8 mol/L) for 2 h-16 h promoted [methylz3H]choline incorporation in time dependence. (2) VIP (10-8 mol/L) enhanced the contents of total phospholipidsand phosphatidylcholine in lung explants. (3) VIP (10-10-10-7 mol/L) elevated microsomal CCT activity of lung explants in dose dependence. (4) VIP (10-8 mol/L) increased expression of CCTα mRNA in lung explants and alveolar type Ⅱ cells (ATII). (5) [D-P-Cl-Phe(6)-Leu(17)]-VIP (10-6 mol/L), a VIP receptors antagonist, abolished the increase of [3H]choline incorporation, microsomal CCT activity and CCTα mRNA level induced by VIP (10-8 mol/L) in lung explants. CONCLUSION: VIP could enhance synthesis of phosphatidylcholine, the major component of pulmonary surfactants by enhancing microsomal CCT activity and CCTα mRNA level via VIP receptormediated pathway.
其他文献
AIM: To investigate the tissue distribution of bitespiramycin (BSPM) and spiramycin (SPM) in rats. METHODS:Liquid chromatographic-mass spectrometric assay was a
Aim:To investigate the influence of resveratrol (Res),a nutritional antioxidant,on the inhibition of apoptosis in rat primary neuron cultures. Methods:The cultu
与栽培种甘蓝型油菜相比，芥菜型油菜具有许多优良遗传特性，如：角果数多，黄籽类型多，含量油量高(45％以上)、抗病、抗倒伏等特点。通过芥甘种间杂交能获得含有某些芥菜型油菜优良基因的种间杂种后代。本研究以芥菜型油菜四川黄籽自交系和甘蓝型油菜Stellar以及甘蓝型油菜自交系1047为亲本进行复交，产生的种间杂种后代自交系BC_1F_6、BC_1F_7为材料进行农艺性状的观察和品质性状的测定，同时利用R
AIM: To clone the cDNA of a new member of snake venom C-type lectin-like proteins, to study its structurefunction relationships and to achieve its recombinant p
AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function
AIM: To study the effects of centrophenoxine (CPH, meclofenoxate) on chronic cerebral hypoperfusion induced deficits in rats. METHODS: Chronic hypoperfusion in
Aim:To investigate the underlying mechanism of acid-sensing ion channel (ASIC) la involved in the acidosis-induced cytotoxicity of rat C6 glioma cells. Methods:
谷子(Setaria italica Beauv)是我国北方干旱和半干旱地区传统的优势农作物之一，营养价值较高，利用杂种优势对提高谷子的产量、改善谷子品质和增强谷子抗逆性具有重要作用。但
试验用辽宁省11个主推杂交粳稻品种(组合)及其亲本共24份材料，对其茎秆抗倒伏特性的形态、生理、内部组织结构和化学组成成分进行了研究。利用灰色关联度方法分析了各个性状之
AIM: To investigate the effects of gap junction inhibitors on endothelium-derived but nitric oxide (NO)- and prostacyclin (PGI2)-independent vasodilatations ind