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目的研究不同时间点旋毛虫肠道急性感染后外周血及肠道黏膜内调节T细胞(CD 4+/CD 25+Treg)的变化规律。方法研究分为旋毛虫急性感染组(感染后2周,4周及8周)、对照组(与感染组相应年龄及饲养环境)。测定肠道病理损伤程度,外周血中及肠道黏膜固有层淋巴细胞中CD 4+/CD 25+T细胞占CD 4+淋巴细胞的百分比。结果 (1)模型动物感染后2周肠道各部位炎症评分明显高于正常组(回肠末端2.85±0.22,近端结肠2.66±0.27,远端结肠2.43±0.19,P<0.05);4周组炎症评分较2周组降低,但仍高于正常组;至感染后8周大致恢复正常。(2)感染2周后外周血Treg百分比明显下降(3.91±0.15 vs 2.28±0.13,P<0.01),下降比例41.7%。造模后4周,外周血Treg百分比仍低于正常对照组(3.91±0.15 vs 3.01±0.14,P<0.01)。而至模型诱导成功的8周后,血Treg的比例(CD 4+CD 25+/CD 4+)趋于正常(3,48±0.15,0.010,05)。结论旋毛虫急性感染诱导感染后肠易激综合征模型中,调节T细胞水平下降。Treg的参与可能与PI-IBS的发生有关。
Objective To study the changes of regulatory T cells (CD 4 + / CD 25 + Treg) in peripheral blood and intestinal mucosa of Trichinella spiralis after acute intestinal infection at different time points. Methods The study was divided into acute infection group (2 weeks, 4 weeks and 8 weeks after infection), control group (corresponding to infection group and feeding environment). The degree of intestinal pathological damage was determined. The percentage of CD 4 + / CD 25 + T cells in CD 4+ lymphocytes in peripheral blood and intestinal lamina propria lymphocytes. Results (1) Inflammatory score of various parts of intestinal tract in model animals at 2 weeks after infection was significantly higher than that in normal group (2.85 ± 0.22 at terminal ileum, 2.66 ± 0.27 at proximal colon, 2.43 ± 0.19 at distal colon, P <0.05) Inflammation score was lower than that of the 2-week group, but still higher than that of the normal group; it returned to normal after 8 weeks of infection. (2) The percentage of Treg in peripheral blood decreased significantly after 2 weeks of infection (3.91 ± 0.15 vs 2.28 ± 0.13, P <0.01), with a decrease of 41.7%. At 4 weeks after modeling, the percentage of Treg in peripheral blood was still lower than that in the normal control group (3.91 ± 0.15 vs 3.01 ± 0.14, P <0.01). After 8 weeks of successful model induction, the proportion of blood Tregs (CD 4 + CD 25 + / CD 4+) tended to be normal (3,48 ± 0.15, 0.01
0.05). Conclusion Trichinella spiralis acute infection induced irritable bowel syndrome model, the regulation of T cell levels decreased. The involvement of Treg may be related to the occurrence of PI-IBS.